Nanopore adaptive sampling for targeted mitochondrial genome sequencing and bloodmeal identification in hematophagous insects.

ABSTRACT:

Background

Blood-feeding insects are important vectors for an array of zoonotic pathogens. While previous efforts toward generating molecular resources have largely focused on major vectors of global medical and veterinary importance, molecular data across a large number of hematophagous insect taxa remain limited. Advancements in long-read sequencing technologies and associated bioinformatic pipelines provide new opportunities for targeted sequencing of insect mitochondrial (mt) genomes. For engorged hematophagous insects, such technologies can be leveraged for both insect mitogenome genome assembly and identification of vertebrate blood-meal sources.

Methods

We used nanopore adaptive sampling (NAS) to sequence genomic DNA from four species of field-collected, blood-engorge

SUBMITTER: Kipp EJ 

PROVIDER: S-EPMC9930342 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature