The 5'UTR of HCoV-OC43 adopts a topologically constrained structure to intrinsically repress translation.

ABSTRACT: The emergence of SARS-CoV-2, which is responsible for the COVID-19 pandemic, has highlighted the need for rapid characterization of viral mechanisms associated with cellular pathogenesis. Viral UTRs represent conserved genomic elements that contribute to such mechanisms. Structural details of most CoV UTRs are not available, however. Experimental approaches are needed to allow for the facile generation of high-quality viral RNA tertiary structural models, which can facilitate comparative mechanistic efforts. By integrating experimental and computational techniques, we herein report the efficient characterization of conserved RNA structures within the 5'UTR of the HCoV-OC43 genome, a lab-tractable model coronavirus. We provide evidence that the 5'UTR folds into a structure with well-defined

SUBMITTER: Mackeown M 

PROVIDER: S-EPMC9930382 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature