Influence of Asp Isomerization on Trypsin and Trypsin-like Proteolysis.

ABSTRACT: Long-lived proteins (LLPs), although less common than their short-lived counterparts, are increasingly recognized to play important roles in age-related diseases such as Alzheimer's. In particular, spontaneous chemical modifications can accrue over time that serve as both indicators of and contributors to disrupted autophagy. For example, isomerization in LLPs is common and occurs in the absence of protein turnover while simultaneously interfering with the protein turnover by impeding proteolysis. In addition to the biological implications this creates, isomerization may also interfere with its own analysis. To clarify, bottom-up proteomics experiments rely on protein digestion by proteases, most commonly trypsin, but the extent to which isomerization might interfere with trypsin digestion

SUBMITTER: Silzel JW 

PROVIDER: S-EPMC9930443 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature