SWR1 chromatin remodeling complex prevents mitotic slippage during spindle position checkpoint arrest.

ABSTRACT: Faithful chromosome segregation in budding yeast requires correct positioning of the mitotic spindle along the mother to daughter cell polarity axis. When the anaphase spindle is not correctly positioned, a surveillance mechanism, named as the spindle position checkpoint (SPOC), prevents the progression out of mitosis until correct spindle positioning is achieved. How SPOC works on a molecular level is not well understood. Here we performed a genome-wide genetic screen to search for components required for SPOC. We identified the SWR1 chromatin-remodeling complex (SWR1-C) among several novel factors that are essential for SPOC integrity. Cells lacking SWR1-C were able to activate SPOC upon spindle misorientation but underwent mitotic slippage upon prolonged SPOC arrest. This mitotic slippa

SUBMITTER: Caydasi AK 

PROVIDER: S-EPMC9930528 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature