An improved method to efficiently acquire rice black-streaked dwarf virus viruliferous small brown planthoppers.

ABSTRACT: Accurate phenotypic identification is the basis of research for resistance genetics and rice breeding for resistance to RBSDV disease. Obtaining rice black-streaked dwarf virus (RBSDV) viruliferous small brown planthoppers (SBPHs) with high transmission efficiency is an essential part of accurate phenotypic identification. Here, through quantifying number of RBSDV copies in infected rice plants, optimizing times of SBPHs fed on RBSDV-infected rice plants and leaf stage of rice seedlings, a method to acquire an RBSDV-carrying SBPH population more efficiently was improved. The results showed that rate of viruliferous SBPHs was significantly higher when fed on RBSDV-infected rice plants that had the copy numbers of RBSDV S10 of 3.0*10⁴ and 1.1*10⁴ than 8.3*10²

SUBMITTER: Du L 

PROVIDER: S-EPMC9930603 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature