GLIS1 intervention enhances anti-PD1 therapy for hepatocellular carcinoma by targeting SGK1-STAT3-PD1 pathway.

ABSTRACT:

Background

GLI-similar 1 (GLIS1) is one of of Krüppel-like zinc finger proteins, which are either stimulators or inhibitors of genetic transcription. Nevertheless, its effects on T cell were elusive.

Methods

In this study, we intend to explore the effects of GLIS1 on modulating the anticancer potency of CD8⁺ T cells in hepatocellular carcinoma (HCC). The expression of GLIS1 in CD8 peripheral blood mononuclear cell and CD8 tumor-infiltrating lymphocytes of HCC tissues was validated by quantificational real-time-PCR and flow cytometry. The anticancer potency of CD8⁺ T cells with GLIS1 knock down was confirmed in C57BL/6 mouse model and HCC patient-derived xenograft mice model. GLIS1^-/- C57BL/6 mice was applied to explore the effects GLIS1 on tu

SUBMITTER: Rong D 

PROVIDER: S-EPMC9930610 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature