Gene expression analysis and the risk of relapse in favorable histology Wilms' tumor.

ABSTRACT:

Introduction and objectives

Wilms' tumor (WT) relapse occurs in 15% of patients. We aim to investigate the association between the expression of several genetic markers and WT relapse risk.

Materials and methods

The study included 51 children treated for WT at a tertiary center between 2001 and 2019: 23 patients had disease relapse (group A) and 28 remained relapse-free after at least 2 years of follow-up (group B). Patients with syndromic, bilateral synchronous or anaplastic WT were excluded. Autologous renal tissue from 20 patients served as control. Total RNA was isolated from tumor tissue and control. Gene expression levels of WT1, HIF1α, b-FGF, c-MYC and SLC22A18 were assessed using quantitative RT-PCR and normalized to GAPDH. Immunohistochemical staining for WT1 and ge

SUBMITTER: Abdel-Monem MM 

PROVIDER: S-EPMC9930804 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature