Thermodynamic origins of two-component multiphase condensates of proteins.

ABSTRACT: Intracellular condensates are highly multi-component systems in which complex phase behaviour can ensue, including the formation of architectures comprising multiple immiscible condensed phases. Relying solely on physical intuition to manipulate such condensates is difficult because of the complexity of their composition, and systematically learning the underlying rules experimentally would be extremely costly. We address this challenge by developing a computational approach to design pairs of protein sequences that result in well-separated multilayered condensates and elucidate the molecular origins of these compartments. Our method couples a genetic algorithm to a residue-resolution coarse-grained protein model. We demonstrate that we can design protein partners to form multiphase conden

SUBMITTER: Chew PY 

PROVIDER: S-EPMC9931050 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature