N6-methyladenosine demethylase FTO enhances chemo-resistance in colorectal cancer through SIVA1-mediated apoptosis.

ABSTRACT: N6-methyladenosine (m⁶A) is the most pervasive RNA modification and is recognized as a novel epigenetic regulation in RNA metabolism. Although the m⁶A modification involves various physiological processes, its roles in drug resistance in colorectal cancer (CRC) still remain unknown. We analyzed the RNA expression profile of m⁶A/A (%) with MRM mass spectrometry in human 5-fluorouracil (5-FU)-resistant CRC tissues, and used the m⁶A RNA immunoprecipitation assay to validate the m⁶A-regulated target. Our results have shown that the m⁶A demethylase FTO was up-regulated in human primary and 5-FU-resistant CRC. Depletion of FTO decreased cell growth, colony formation and metastasis in 5-FU-resistant CRC cells in vitro and in vivo.

SUBMITTER: Lin Z 

PROVIDER: S-EPMC9931553 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature