A competitive precision CRISPR method to identify the fitness effects of transcription factor binding sites.

ABSTRACT: Here we describe a competitive genome editing method that measures the effect of mutations on molecular functions, based on precision CRISPR editing using template libraries with either the original or altered sequence, and a sequence tag, enabling direct comparison between original and mutated cells. Using the example of the MYC oncogene, we identify important transcriptional targets and show that E-box mutations at MYC target gene promoters reduce cellular fitness.

SUBMITTER: Pihlajamaa P 

PROVIDER: S-EPMC9931575 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature