Ontology highlight
ABSTRACT:
SUBMITTER: Borm LE
PROVIDER: S-EPMC9931581 | biostudies-literature | 2023 Feb
REPOSITORIES: biostudies-literature
Borm Lars E LE Mossi Albiach Alejandro A Mannens Camiel C A CCA Janusauskas Jokubas J Özgün Ceren C Fernández-García David D Hodge Rebecca R Castillo Francisca F Hedin Charlotte R H CRH Villablanca Eduardo J EJ Uhlén Per P Lein Ed S ES Codeluppi Simone S Linnarsson Sten S
Nature biotechnology 20220922 2
Methods to spatially profile the transcriptome are dominated by a trade-off between resolution and throughput. Here we develop a method named Enhanced ELectric Fluorescence in situ Hybridization (EEL FISH) that can rapidly process large tissue samples without compromising spatial resolution. By electrophoretically transferring RNA from a tissue section onto a capture surface, EEL speeds up data acquisition by reducing the amount of imaging needed, while ensuring that RNA molecules move straight ...[more]