Cell type matters: competence for alkaloid metabolism differs in two seed-derived cell strains of Catharanthus roseus.

ABSTRACT: Since the discovery of the anticancer drugs vinblastine and vincristine, Catharanthus roseus has been intensively studied for biosynthesis of several terpene indole alkaloids (TIAs). Due to their low abundance in plant tissues at a simultaneously high demand, modes of production alternative to conventional extraction are mandatory. Plant cell fermentation might become one of these alternatives, yet decades of research have shown limited success to certain product classes, leading to the question: how to preserve the intrinsic ability to produce TIAs (metabolic competence) in cell culture? We used the strategy to use the developmental potency of mature embryos to generate such strains. Two cell strains (C1and C4) from seed embryos of Catharanthus roseus were found to differ not only morphol

SUBMITTER: Raorane ML 

PROVIDER: S-EPMC9931846 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature