Detection of carrier Booroola (Fec^B) allele in BMPR1B gene of MEGA (Merino × Garut) sheep and its association with growth traits.

ABSTRACT:

Background

Bone morphogenetic protein receptor 1B (BMPR1B) gene is one of candidate genes for reproductive and growth traits in sheep. The present study was aimed to detect the Booroola (Fec^B) allele in BMPR1B gene and its association with growth traits in MEGA (Merino × Garut) sheep. A total of 82DNA samples collected from individual lamb (mixed-sex) blood were genotyped for allelic polymorphism using a PCR-RFLP method.

Results

The PCR analysis in BMPR1B gene resulted the amplicons with size of140 bp. The RFLP analysis with AvaII restriction enzymeresultedtwo allelic types of wildtype (A/Fec⁺) and mutant or Booroola (G/Fec^B) with frequency of 0.89 and 0.11, respectively. However, the genetic diversity in BMPR1B/AvaII gene of animal studies w

SUBMITTER: Margawati ET 

PROVIDER: S-EPMC9931984 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature