NSUN2 promotes osteosarcoma progression by enhancing the stability of FABP5 mRNA via m⁵C methylation.

ABSTRACT: 5-methylcytosine (m⁵C) modification, which is mainly induced by the RNA methyltransferase NSUN2 (NOP2/Sun domain family, member 2), is an important chemical posttranscriptional modification in mRNA and has been proven to play important roles in the progression of many cancers. However, the functions and underlying molecular mechanisms of NSUN2-mediated m⁵C in osteosarcoma (OS) remain unclear. In this study, we found NSUN2 was highly expressed in OS tissues and cells. We also discovered that higher expression of NSUN2 predicted poorer prognosis of OS patients. Our study showed that NSUN2 could promote the progression of OS cells. Moreover, we employed RNA sequencing, RNA immunoprecipitation (RIP), and methylated RIP to screen and validate the candidate targets of NSUN2

SUBMITTER: Yang M 

PROVIDER: S-EPMC9932088 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature