Cholesterol accumulation in ovarian follicles causes ovulation defects in Abca1a ^-/- Japanese medaka (Oryzias latipes).

ABSTRACT: ATP-binding cassette A1 (ABCA1) is a membrane protein, which exports excess cellular cholesterol to generate HDL to reduce the risk of the onset of cardiovascular diseases (CVD). In addition, ABCA1 exerts pleiotropic effects on such as inflammation, tissue repair, and cell proliferation and migration. In this study, we explored the novel physiological roles of ABCA1 using Japanese medaka (Oryzias latipes), a small teleost fish. Three Abca1 genes were found in the medaka genome. ABCA1A and ABCA1C exported cholesterol to generate nascent HDL as human ABCA1 when expressed in HEK293 cells. To investigate their physiological roles, each Abca1-deficient fish was generated using the CRISPR-Cas9 system. Abca1a ^-/- female medaka was found to be infertil

SUBMITTER: Futamata R 

PROVIDER: S-EPMC9932449 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature