Bioinformatics analysis identifies heparan sulfate proteoglycans acting as different progress subtypes of biliary atresia.

ABSTRACT:

Background

Biliary atresia (BA) is a life-threatening disorder, which is characterized by the obliteration of biliary tracts. Heparan sulfate proteoglycans (HSPGs) are important regulators in liver diseases. Whether HSPGs participate in the development of BA is poorly understood.

Methods

RNA-seq dataset GSE122340, including 171 BA and 7 normal liver tissue, was integrated for bioinformatic analysis. R function "wilcox.test" was used to compare HSPGs expression levels, and "cor.test" was used to evaluate the correlation analysis. MCPcounter was used to assess the abundance of immunocytes. Molecular subtypes of BA were clustered via NMF clustering and LASSO regression was applied to screen hub HSPGs genes in BA clusters. RT-PCR analysis was used to assess the expression

SUBMITTER: Ding Z 

PROVIDER: S-EPMC9932896 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature