Optimized workflow to modify microRNA expression in primary human intravascular cells.

ABSTRACT:

Background

A comprehensive dissection of the role of microRNAs (miRNAs) in gene regulation and subsequent cell functions requires a specific and efficient knockdown or overexpression of the miRNA of interest; these are achieved by transfecting the cell of interest with a miRNA inhibitor or a miRNA mimic, respectively. Inhibitors and mimics of miRNAs with a unique chemistry and/or structural modifications are available commercially and require different transfection conditions. Here, we aimed to investigate how various conditions affect the transfection efficacy of two miRNAs with high and low endogenous expression, miR-15a-5p and miR-20b-5p respectively, in human primary cells.

Results

MiRNA inhibitors and mimics from two commonly used commercial vendors were employed, i.e.,

SUBMITTER: Caglayan S 

PROVIDER: S-EPMC9933393 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature