ASAP─Automated Sonication-Free Acid-Assisted Proteomes─from Cells and FFPE Tissues.

ABSTRACT: Formalin-fixed, paraffin-embedded (FFPE) tissues are an invaluable resource for retrospective studies, but protein extraction and subsequent sample processing steps have been shown to be challenging for mass spectrometry (MS) analysis. Streamlined high-throughput sample preparation workflows are essential for efficient peptide extraction from complex clinical specimens such as fresh frozen tissues or FFPE. Overall, proteome analysis has gained significant improvements in the instrumentation, acquisition methods, sample preparation workflows, and analysis pipelines, yet even the most recent FFPE workflows remain complex and are not readily scalable. Here, we present an optimized workflow for automated sonication-free acid-assisted proteome (ASAP) extraction from FFPE sections. ASAP enables

SUBMITTER: Barnabas GD 

PROVIDER: S-EPMC9933881 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature