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Using the Culex pipiens sperm proteome to identify elements essential for mosquito reproduction.

ABSTRACT: Mature sperm from Culex pipiens were isolated and analyzed by mass spectrometry to generate a mature sperm proteome dataset. In this study, we highlight subsets of proteins related to flagellar structure and sperm motility and compare the identified protein components to previous studies examining essential functions of sperm. The proteome includes 1700 unique protein IDs, including a number of uncharacterized proteins. Here we discuss those proteins that may contribute to the unusual structure of the Culex sperm flagellum, as well as potential regulators of calcium mobilization and phosphorylation pathways that regulate motility. This database will prove useful for understanding the mechanisms that activate and maintain sperm motility as well as identify potential molecular targets for mosquito population control.

SUBMITTER: Thaler CD

PROVIDER: S-EPMC9934393 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature

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Using the Culex pipiens sperm proteome to identify elements essential for mosquito reproduction.

Thaler Catherine D CD Carstens Kaira K Martinez Gabrielle G Stephens Kimberly K Cardullo Richard A RA

PloS one 20230216 2

Mature sperm from Culex pipiens were isolated and analyzed by mass spectrometry to generate a mature sperm proteome dataset. In this study, we highlight subsets of proteins related to flagellar structure and sperm motility and compare the identified protein components to previous studies examining essential functions of sperm. The proteome includes 1700 unique protein IDs, including a number of uncharacterized proteins. Here we discuss those proteins that may contribute to the unusual structure ...[more]

PMID: 36795667

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