Neural progenitor cell-derived extracellular matrix as a new platform for neural differentiation of human induced pluripotent stem cells.

ABSTRACT: The culture microenvironment has been demonstrated to regulate stem cell fate and to be a crucial aspect for quality-controlled stem cell maintenance and differentiation to a specific lineage. In this context, extracellular matrix (ECM) proteins are particularly important to mediate the interactions between the cells and the culture substrate. Human induced pluripotent stem cells (hiPSCs) are usually cultured as anchorage-dependent cells and require adhesion to an ECM substrate to support their survival and proliferation in vitro. Matrigel, a common substrate for hiPSC culture is a complex and undefined mixture of ECM proteins which are expensive and not well suited to clinical application. Decellularized cell-derived ECM has been shown to be a promising alternative to the common pr

SUBMITTER: Carvalho MS 

PROVIDER: S-EPMC9934470 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature