Application of bioengineered elastin-like polypeptide-based system for targeted gene delivery in tumor cells.

ABSTRACT: Successful gene delivery depends on the entry of negatively charged DNAs and oligonucleotides across the various barriers of the tumor cells and localization into the nucleus for its transcription and protein translation. Here, we have reported a thermal responsive self-assemble and highly biocompatible, targeted ELP-based gene delivery system. These systems consist of cell-penetrating peptides, Tat and single or multiple repeats of IL-4 receptor targeting peptide AP-1 along the backbone of ELP. Cell-penetrating peptides were introduced for nuclear localization of genes of interest, AP-1 for targeting IL-4R highly expressed tumor cells and ELP for stable condensation favoring protection of nucleic acids. The designed multidomain fusion ELPs referred to as Tat-ELP, Tat-A₁E₂₈

SUBMITTER: Yi A 

PROVIDER: S-EPMC9934475 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature