Growth factor and macromolecular crowding supplementation in human tenocyte culture.

ABSTRACT: Cell-assembled tissue engineering strategies hold great potential in regenerative medicine, as three-dimensional tissue-like modules can be produced, even from a patient's own cells. However, the development of such implantable devices requires prolonged in vitro culture time, which is associated with cell phenotypic drift. Considering that the cells in vivo are subjected to numerous stimuli, multifactorial approaches are continuously gaining pace towards controlling cell fate during in vitro expansion. Herein, we assessed the synergistic effect of simultaneous and serial growth factor supplementation (insulin growth factor-1, platelet-derived growth factor ββ, growth differentiation factor 5 and transforming growth factor β3) to macromolecular crowding (

SUBMITTER: Tsiapalis D 

PROVIDER: S-EPMC9934496 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature