DNA release from plant tissue using focused ultrasound extraction (FUSE).

ABSTRACT:

Premise

Sample preparation in genomics is a critical step that is often overlooked in molecular workflows and impacts the success of downstream genetic applications. This study explores the use of a recently developed focused ultrasound extraction (FUSE) technique to enable the rapid release of DNA from plant tissues for genetic analysis.

Methods

FUSE generates a dense acoustic cavitation bubble cloud that pulverizes targeted tissue into acellular debris. This technique was applied to leaf samples of American chestnut (Castanea dentata), tulip poplar (Liriodendron tulipifera), red maple (Acer rubrum), and chestnut oak (Quercus montana).

Results

We observed that FUSE can extract high quantities of DNA in 9-15 min, compared to the 30 min requ

SUBMITTER: Stettinius A 

PROVIDER: S-EPMC9934592 | biostudies-literature | 2023 Jan-Feb

REPOSITORIES: biostudies-literature