Fasting and refeeding triggers specific changes in bile acid profiles and gut microbiota.

ABSTRACT:

Background

Bile acids (BAs) are closely related to nutrient supply and modified by gut microbiota. Gut microbiota perturbations shape BA composition, which further affects host metabolism.

Methods

We investigated BA profiles in plasma, feces, and liver of mice fed ad libitum, fasted for 24 h, fasted for 24 h and then refed for 24 h using ultraperformance liquid chromatography coupled to tandem mass spectrometry. Gut microbiota was measured by 16S rRNA gene sequencing. Expressions of BA biosynthesis-related genes in the liver and BA reabsorption-related genes in the ileum were analyzed.

Findings

Compared with the controls, unconjugated primary BAs (PBAs) and unconjugated secondary BAs (SBAs) in plasma were decreased whereas conjugated SBAs in plasma, unconjugated PBAs,

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC9934961 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature