Immunohistochemical characteristics of local sites that trigger atrial arrhythmias in response to high-frequency stimulation.

ABSTRACT:

Aims

The response to high frequency stimulation (HFS) is used to locate putative sites of ganglionated plexuses (GPs), which are implicated in triggering atrial fibrillation (AF). To identify topological and immunohistochemical characteristics of presumed GP sites functionally identified by HFS.

Methods and results

Sixty-three atrial sites were tested with HFS in four Langendorff-perfused porcine hearts. A 3.5 mm tip quadripolar ablation catheter was used to stimulate and deliver HFS to the left and right atrial epicardium, within the local atrial refractory period. Tissue samples from sites triggering atrial ectopy/AF (ET) sites and non-ET sites were stained with choline acetyltransferase (ChAT) and tyrosine hydroxylase (TH), for quantification of parasympathetic and sympat

SUBMITTER: Kim MY 

PROVIDER: S-EPMC9935019 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature