Fluorescence in situ hybridization and polymerase chain reaction to detect infections of cardiac implantable electronic devices.

ABSTRACT:

Aims

In patients with infections of cardiac implantable electronic devices (CIEDs), the identification of causative pathogens is complicated by biofilm formations and previous antibiotic therapy. In this work, the impact of an additional fluorescence in situ hybridization (FISH), in combination with polymerase chain reaction and sequencing (FISHseq) was investigated.

Methods and results

In 36 patients with CIED infections, FISHseq of explanted devices was performed and compared with standard microbiological cultivation of preoperative and intraoperative samples. The mean age was 61.9 (±16.2) years; 25 (69.4%) were males. Most patients (62.9%) had heart failure with reduced ejection fraction. Infections occurred as endoplastits (n = 26), isolated local generator pocket infect

SUBMITTER: Just IA 

PROVIDER: S-EPMC9935028 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature