Effect of chitooligosaccharide on the inhibition of SARS-CoV-2 main protease.

ABSTRACT:

Background

The main protease (Mpro) is a crucial target for severe acute respiratory syndrome coronavirus (SARS-CoV-2). Chitooligosaccharide (CS) has broad-spectrum antiviral activity and can effectively inhibit the activity of SARS-CoV. Here, based on the high homology between SARS-CoV-2 and SARS-CoV, this study explores the effect and mechanism of CS with various molecular weights on the activity of SARS-CoV-2 Mpro.

Methods

We used fluorescence resonance energy transfer (FRET), UV-Vis, synchronous fluorescence spectroscopy, circular dichroism (CD) spectroscopy and computational simulation to investigate the molecular interaction and the interaction mechanism between CS and SARS-CoV-2 Mpro.

Results

Four kinds of CS with different molecular weights significantly inhib

SUBMITTER: Wang Q 

PROVIDER: S-EPMC9935244 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature