Enzymatically amplified linear dbDNA^TM as a rapid and scalable solution to industrial lentiviral vector manufacturing.

ABSTRACT: Traditional bacterial fermentation techniques used to manufacture plasmid are time-consuming, expensive, and inherently unstable. The production of sufficient GMP grade material thus imposes a major bottleneck on industrial-scale manufacturing of lentiviral vectors (LVV). Touchlight's linear doggybone DNA (dbDNA^TM) is an enzymatically amplified DNA vector produced with exceptional speed through an in vitro dual enzyme process, enabling industrial-scale manufacturing of GMP material in a fraction of the time required for plasmid. We have previously shown that dbDNA^TM can be used to produce functional LVV; however, obtaining high LVV titres remained a challenge. Here, we aimed to demonstrate that dbDNA^TM could be optimised for the manufacture of high titre LV

SUBMITTER: Barreira M 

PROVIDER: S-EPMC9935383 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature