Optimized protocol for MALDI MSI of N-glycans using an on-tissue digestion in fresh frozen tissue sections.

ABSTRACT: Glycans play an important role in biology with multiple cellular functions ranging from cell signaling, mobility and growth to protein folding and localization. The N-glycosylation state within a tissue has been found to vary greatly between healthy and diseased patients and has proven to have an important clinical diagnostic value. Matrix assisted laser-desorption ionization (MALDI) mass spectrometry imaging (MSI) allows for untargeted analysis of biomolecules, including N-glycans, on a tissue section and provides a spatial context of the analyte. Until now, N-glycans have been predominantly analyzed using MALDI MSI on formalin-fixed paraffin embedded (FFPE) tissue sections, however this greatly reduces the clinical applicability, as the FFPE embedding process alters the biological enviro

SUBMITTER: Grgic A 

PROVIDER: S-EPMC9935634 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature