Identification of p72 epitopes of African swine fever virus and preliminary application.

ABSTRACT: African swine fever virus (ASFV) causes a highly lethal hemorrhagic viral disease (ASF) of pigs that results in serious losses in China and elsewhere. The development of a vaccine and diagnosis technology for ASFV is essential to prevent and control the spread of ASF. The p72 protein of ASFV is highly immunogenic and reactive, and is a dominant antigen in ASF vaccine and diagnostic research. In this study, 17 p72 monoclonal antibodies (mAbs) were generated. Epitope mapping by a series of overlapping peptides expressed in Escherichia coli showed that these mAbs recognized a total of seven (1-7) linear B cell epitopes. These mAbs did not show significant neutralizing activity. Epitopes 1 (²⁴⁹HKPHQSKPIL²⁵⁸), 2 (⁶⁹PVGFEYENKV⁷⁷), 5 (¹⁹⁵

SUBMITTER: Miao C 

PROVIDER: S-EPMC9935695 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature