An miR156-regulated nucleobase-ascorbate transporter 2 confers cadmium tolerance via enhanced anti-oxidative capacity in barley.

ABSTRACT:

Introduction

Cadmium (Cd) is one of the most detrimental heavy metal pollutants, seriously affecting crop production and human health. Nucleobase-ascorbic acid transporters (NAT) are widely present in many living organisms including plants, animals and microbes; however, the role of NAT in plant Cd tolerance remains unknown.

Objectives

To identify Cd-induced miRNAs that target HvNAT2 and to determine the role of this gene and its product in Cd tolerance.

Methods

High-throughput-sequencing was used to identify the miRNA expression profile of barley roots in response to Cd stress. Overexpression (OX) and RNAi lines were then constructed for HvNAT2 and comparative transcriptomic analysis was performed to determine the function of this transporter examining its effects on

SUBMITTER: Wang NH 

PROVIDER: S-EPMC9936425 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature