Azobenzene-Based Amino Acids for the Photocontrol of Coiled-Coil Peptides.

ABSTRACT: Coiled-coil peptides are high-affinity, selective, self-assembling binding motifs, making them attractive components for the preparation of functional biomaterials. Photocontrol of coiled-coil self-assembly allows for the precise localization of their activity. To rationally explore photoactivity in a model coiled coil, three azobenzene-containing amino acids were prepared and substituted into the hydrophobic core of the E₃/K₃ coiled-coil heterodimer. Two of the non-natural amino acids, APhe1 and APhe2, are based on phenylalanine and differ in the presence of a carboxylic acid group. These have previously been demonstrated to modulate protein activity. When incorporated into peptide K₃, coiled-coil binding strength was affected upon isomerizat

SUBMITTER: Crone NSA 

PROVIDER: S-EPMC9936496 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature