Resolving Molecular Heterogeneity with Single-Molecule Centrifugation.

ABSTRACT: For many classes of biomolecules, population-level heterogeneity is an essential aspect of biological function─from antibodies produced by the immune system to post-translationally modified proteins that regulate cellular processes. However, heterogeneity is difficult to fully characterize for multiple reasons: (i) single-molecule approaches are needed to avoid information lost by ensemble-level averaging, (ii) sufficient statistics must be gathered on both a per-molecule and per-population level, and (iii) a suitable analysis framework is required to make sense of a potentially limited number of intrinsically noisy measurements. Here, we introduce an approach that overcomes these difficulties by combining three techniques: a DNA nanoswitch construct to repeatedly interrogate the same mole

SUBMITTER: Luo Y 

PROVIDER: S-EPMC9936575 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature