The transgenic IG-DMR sequence of the mouse Dlk1-Dio3 domain acquired imprinted DNA methylation during the post-fertilization period.

ABSTRACT:

Background

Allele-specific methylation of the imprinting control region (ICR) is the molecular basis for the genomic imprinting phenomenon that is unique to placental mammals. We previously showed that the ICR at the mouse H19 gene locus (H19 ICR) was unexpectedly established after fertilization and not during spermatogenesis in transgenic mice (TgM), and that the same activity was essential for the maintenance of paternal methylation of the H19 ICR at the endogenous locus in pre-implantation embryos. To examine the universality of post-fertilization imprinted methylation across animal species or imprinted loci, we generated TgM with two additional sequences.

Results

The rat H19 ICR, which is very similar in structure to the mouse H19 ICR, unexpectedly did not acquire imprin

SUBMITTER: Matsuzaki H 

PROVIDER: S-EPMC9936741 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature