Interactive nanocluster compaction of the ELKS scaffold and Cacophony Ca²⁺ channels drives sustained active zone potentiation.

ABSTRACT: At presynaptic active zones (AZs), conserved scaffold protein architectures control synaptic vesicle (SV) release by defining the nanoscale distribution and density of voltage-gated Ca²⁺ channels (VGCCs). While AZs can potentiate SV release in the minutes range, we lack an understanding of how AZ scaffold components and VGCCs engage into potentiation. We here establish dynamic, intravital single-molecule imaging of endogenously tagged proteins at Drosophila AZs undergoing presynaptic homeostatic potentiation. During potentiation, the numbers of α1 VGCC subunit Cacophony (Cac) increased per AZ, while their mobility decreased and nanoscale distribution compacted. These dynamic Cac changes depended on the interaction between Cac channel's intracellular carboxyl terminus and

SUBMITTER: Ghelani T 

PROVIDER: S-EPMC9937578 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature