A Microfluidic Device for Long-Term Maintenance of Organotypic Liver Cultures.

ABSTRACT: Liver cultures may be used for disease modeling, testing therapies and predicting drug-induced injury. The complexity of the liver cultures has evolved from hepatocyte monocultures to co-cultures with non-parenchymal cells and finally to precision-cut liver slices. The latter culture format retains liver's native biomolecular and cellular complexity and therefore holds considerable promise for in vitro testing. However, liver slices remain functional for ~72 h in vitro and display limited utility for some disease modeling and therapy testing applications that require longer culture times. This paper describes a microfluidic device for longer-term maintenance of functional organotypic liver cultures. Our microfluidic culture system was designed to enable direct injection of liver tissue int

SUBMITTER: Vega JMH 

PROVIDER: S-EPMC9937715 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature