Dataset Information

Silencing circUSP48 suppresses osteosarcoma progression by regulating the miR-335/ smad nuclear interacting protein 1 pathway.

ABSTRACT:

Background

Circular RNAs (circRNAs) can have a critical function in the multi-processes of osteosarcoma (OS). Nevertheless, whether circUSP48 is involved in OS progression remains unclear.

Methods

In the current work, the expression of circUSP48, miR-335 and SNIP1 in OS cell lines and tissues were evaluated using qRT-PCR. Then, Sanger sequencing, RNase R treatment and FISH assay were performed for circUSP48 validation. Furthermore, the function and potential mechanisms of circUSP48 in OS were investigated by performing loss-of-function experiments.

Results

Silencing circUSP48 could suppress proliferation, invasion as well as migration of OS cells in vitro, also inhibiting the growth of tumor in vivo. Importantly, circUSP48 promoted OS malignancy by sponging miR-335 to upregulate SNIP1.

Conclusion

Overall, these findings suggested that circUSP48 acted as an oncogene in OS, which might become a new target for OS therapy.

SUBMITTER: Luo Y

PROVIDER: S-EPMC9937887 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature

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Publications

Silencing circUSP48 suppresses osteosarcoma progression by regulating the miR-335/ smad nuclear interacting protein 1 pathway.

Luo Yue Y Yang Bo B Yuan Xiaopin X Zheng Jian J

Journal of clinical laboratory analysis 20230104 2

<h4>Background</h4>Circular RNAs (circRNAs) can have a critical function in the multi-processes of osteosarcoma (OS). Nevertheless, whether circUSP48 is involved in OS progression remains unclear.<h4>Methods</h4>In the current work, the expression of circUSP48, miR-335 and SNIP1 in OS cell lines and tissues were evaluated using qRT-PCR. Then, Sanger sequencing, RNase R treatment and FISH assay were performed for circUSP48 validation. Furthermore, the function and potential mechanisms of circUSP4 ...[more]

PMID: 36597862

Similar Datasets

Project description:BackgroundOsteosarcoma (OS) is a highly invasive primary malignancy of the bone that is common in children and adolescents. MicroRNAs (miRNAs) are novel diagnostic and predictive biomarkers for cancers. The miRNA miR-3195 is aberrantly expressed in multiple types of tumors. However, the expression levels and biological functions of miR-3195 in OS remain unclear.MethodsTwo Gene Expression Omnibus (GEO) datasets (GSE69470 and GSE16088) were used to analyze differentially expressed miRNAs and mRNAs in osteosarcoma cell lines and OS tissues. Quantitative RT-PCR was used to detect the expression levels of miR-3195 and the SRY-box transcription factor 4 (SOX4) mRNA in OS tissues and cell lines. The relationship between miR-3195 and the 3'-upstream region (3'-UTR) in the SOX4 mRNA (predicted through bioinformatics) was analyzed using Pearson's correlation analysis and confirmed by a dual-luciferase reporter gene experiment. Cell counting kit-8 assays, colony formation assays, flow cytometry, wound healing assays, transwell assays, and western blotting were performed to explore the effects of miR-3195 levels on SOX4 affected OS cell biological behavior.ResultsOur results revealed that miR-3195 was the most down-regulated miRNA and SOX4 was the most up-regulated mRNA by Bioinformatic analysis. It was further confirmed miR-3195 had low expression, and SOX4 had high expression levels in clinical OS tissue samples; the expression levels of both genes were negatively correlated with each other in OS tissues. Overexpression of miR-3195 in OS cell lines significantly inhibited cell proliferation, migration, and invasiveness, while promoting apoptosis; all these effects were reversed by increasing SOX4 expression levels. We also found that miR-3195 could directly bind with the SOX4 gene and down-regulate SOX4 expression.ConclusionsmiR-3195 can modulate proliferation, migration, invasiveness, and apoptosis in OS cells by regulating the SOX4 gene. Thus, the miR-3195/SOX4 signaling may be a novel therapeutic target in OS treatment.

Dataset Information

Silencing circUSP48 suppresses osteosarcoma progression by regulating the miR-335/ smad nuclear interacting protein 1 pathway.

Background

Methods

Results

Conclusion

Publications

Silencing circUSP48 suppresses osteosarcoma progression by regulating the miR-335/ smad nuclear interacting protein 1 pathway.

Similar Datasets

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