Heterologous mogrosides biosynthesis in cucumber and tomato by genetic manipulation.
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ABSTRACT:
SUBMITTER: Liao J
PROVIDER: S-EPMC9938114 | biostudies-literature | 2023 Feb
REPOSITORIES: biostudies-literature
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Heterologous mogrosides biosynthesis in cucumber and tomato by genetic manipulation.
Liao Jingjing J Liu Tingyao T Xie Lei L Mo Changming C Qiao Jing J Huang Xiyang X Cui Shengrong S Jia Xunli X Luo Zuliang Z Ma Xiaojun X
Communications biology 20230217 1
Mogrosides are widely used as high-value natural zero-calorie sweeteners that exhibit an array of biological activities and allow for vegetable flavour breeding by modern molecular biotechnology. In this study, we developed an In-fusion based gene stacking strategy for transgene stacking and a multi-gene vector harbouring 6 mogrosides biosynthesis genes and transformed it into Cucumis sativus and Lycopersicon esculentum. Here we show that transgenic cucumber can produce mogroside V and siamenosi ...[more]
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