CRISPR-Cas provides limited phage immunity to a prevalent gut bacterium in gnotobiotic mice.

ABSTRACT: Many bacteria and archaea harbor the adaptive CRISPR-Cas system, which stores small nucleotide fragments from previous invasions of nucleic acids via viruses or plasmids. This molecular archive blocks further invaders carrying identical or similar nucleotide sequences. However, few of these systems have been confirmed experimentally to be active in gut bacteria. Here, we demonstrate experimentally that the type I-C CRISPR-Cas system of the prevalent gut bacterium Eggerthella lenta can specifically target and cleave foreign DNA in vitro by using a plasmid transformation assay. We also show that the CRISPR-Cas system acquires new immunities (spacers) from the genome of a virulent E. lenta phage using traditional phage assays in vitro but also in vivo using gnotobiotic (GB) mice. Both high ph

SUBMITTER: Rasmussen TS 

PROVIDER: S-EPMC9938214 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature