Phenotypic screening platform identifies statins as enhancers of immune cell-induced cancer cell death.

ABSTRACT:

Background

High-throughput screening (HTS) of small molecule drug libraries has greatly facilitated the discovery of new cancer drugs. However, most phenotypic screening platforms used in the field of oncology are based solely on cancer cell populations and do not allow for the identification of immunomodulatory agents.

Methods

We developed a phenotypic screening platform based on a miniaturized co-culture system with human colorectal cancer- and immune cells, providing a model that recapitulates part of the tumor immune microenvironment (TIME) complexity while simultaneously being compatible with a simple image-based readout. Using this platform, we screened 1,280 small molecule drugs, all approved by the Food and Drug Administration (FDA), and identified statins as enhance

SUBMITTER: Selvin T 

PROVIDER: S-EPMC9938546 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature