Novel DNA methylation biomarkers in stool and blood for early detection of colorectal cancer and precancerous lesions.

ABSTRACT:

Background

Early detection and prevention of precancerous lesions can significantly reduce the morbidity and mortality of colorectal cancer (CRC). Here, we developed new candidate CpG site biomarkers for CRC and evaluated the diagnostic value of their expression in blood and stool samples of CRC and precancerous lesions.

Methods

We analyzed 76 pairs of CRC and adjacent normal tissue samples, 348 stool samples, and 136 blood samples. Candidate biomarkers for CRC were screened using a bioinformatics database and identified using a quantitative methylation-specific PCR method. The methylation levels of the candidate biomarkers were validated using blood and stool samples. The divided stool samples were used to construct and validate a combined diagnostic model and to analyze th

SUBMITTER: Shen Y 

PROVIDER: S-EPMC9938553 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature