Dataset Information

Immune cell landscape in symptomatic and asymptomatic SARS-CoV-2 infected adults and children in urban Dhaka, Bangladesh.

ABSTRACT:

Objectives

The study of cellular immunity to SARS-CoV-2 is crucial for evaluating the course of the COVID-19 disease and for improving vaccine development. We aimed to assess the phenotypic landscape of circulating lymphocytes and mononuclear cells in adults and children who were seropositive to SARS-CoV-2 in the past 6 months.

Methods

Blood samples (n = 350) were collected in a cross-sectional study in Dhaka, Bangladesh (Oct 2020-Feb 2021). Plasma antibody responses to SARS-CoV-2 were determined by an electrochemiluminescence immunoassay while lymphocyte and monocyte responses were assessed using flow cytometry including dimensionality reduction and clustering algorithms.

Results

SARS-CoV-2 seropositivity was observed in 52% of adults (18-65 years) and 56% of children (10-17 years). Seropositivity was associated with reduced CD3⁺T cells in both adults (beta(β) = -2.86; 95% Confidence Interval (CI) = -5.98, 0.27) and children (β = -8.78; 95% CI = -13.8, -3.78). The frequencies of T helper effector (CD4⁺T_EFF) and effector memory cells (CD4⁺T_EM) were increased in seropositive compared to seronegative children. In adults, seropositivity was associated with an elevated proportion of cytotoxic T central memory cells (CD8⁺T_CM). Overall, diverse manifestations of immune cell dysregulations were more prominent in seropositive children compared to adults, who previously had COVID-like symptoms. These changes involved reduced frequencies of CD4⁺T_EFF cells and CD163⁺CD64⁺ classical monocytes, but increased levels of intermediate or non-classical monocytes, as well as CD8⁺T_EM cells in symptomatic children.

Conclusion

Seropositive individuals in convalescence showed increased central and effector memory T cell phenotypes and pro-resolving/healing monocyte phenotypes compared to seronegative subjects. However, seropositive children with a previous history of COVID-like symptoms, displayed an ongoing innate inflammatory trait.

SUBMITTER: Akhtar E

PROVIDER: S-EPMC9938758 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature

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Publications

Immune cell landscape in symptomatic and asymptomatic SARS-CoV-2 infected adults and children in urban Dhaka, Bangladesh.

Akhtar Evana E Mily Akhirunnesa A Sarker Protim P Chanda Bikash Chandra BC Haque Farjana F Kuddusi Rakib Ullah RU Haq Md Ahsanul MA Lourda Magda M Brighenti Susanna S Raqib Rubhana R

Immunobiology 20230218 2

<h4>Objectives</h4>The study of cellular immunity to SARS-CoV-2 is crucial for evaluating the course of the COVID-19 disease and for improving vaccine development. We aimed to assess the phenotypic landscape of circulating lymphocytes and mononuclear cells in adults and children who were seropositive to SARS-CoV-2 in the past 6 months.<h4>Methods</h4>Blood samples (n = 350) were collected in a cross-sectional study in Dhaka, Bangladesh (Oct 2020-Feb 2021). Plasma antibody responses to SARS-CoV-2 ...[more]

PMID: 36822063

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Dataset Information

Immune cell landscape in symptomatic and asymptomatic SARS-CoV-2 infected adults and children in urban Dhaka, Bangladesh.

Objectives

Methods

Results

Conclusion

Publications

Immune cell landscape in symptomatic and asymptomatic SARS-CoV-2 infected adults and children in urban Dhaka, Bangladesh.

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