Insights Into The Effects of Amino Acid Substitutions on The Stability of Reteplase Structure: A Molecular Dynamics Simulation Study.

ABSTRACT:

Background

Reteplase (recombinant plasminogen activator, r-PA) is a recombinant protein designed to imitate the endogenous tissue plasminogen activator and catalyze the plasmin production. It is known that the application of reteplase is limited by the complex production processes and protein's stability challenges. Computational redesign of proteins has gained momentum in recent years, particularly as a powerful tool for improving protein stability and consequently its production efficiency. Hence, in the current study, we implemented computational approaches to improve r-PA conformational stability, which fairly correlates with protein's resistance to proteolysis.

Objectives

The current study was developed in order to evaluate the effect of amino acid substitutions on the

SUBMITTER: Haji-Allahverdipoor K 

PROVIDER: S-EPMC9938932 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature