Lymphatic Drainage System and Lymphatic Metastasis of Cancer Cells in the Mouse Esophagus.

ABSTRACT:

Background

Lymphatic metastasis is commonly seen in patients with esophageal squamous cell carcinoma (ESCC). Both lymphatic metastasis and the number of involved nodes are prognostic for post-operative survival. To better understand lymphatic metastasis of ESCC, there is a need to develop proper animal models.

Aims

This study is aimed to characterize the morphology and function of the lymphatic drainage system in the mouse esophagus.

Methods

Immunostaining and fluorescence imaging were used to visualize the lymphatic drainage system in the mouse esophagus. Tracers and cancer cells were orthotopically inoculated into the submucosa of the mouse esophagus to mimic lymphatic metastasis of T1 ESCC.

Results

Using immunostaining of a lymphatic vessel marker (LYVE1), w

SUBMITTER: Li Y 

PROVIDER: S-EPMC9938949 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature