An interactive web application for processing, correcting, and visualizing genome-wide pooled CRISPR-Cas9 screens.

ABSTRACT: A limitation of pooled CRISPR-Cas9 screens is the high false-positive rate in detecting essential genes arising from copy-number-amplified genomics regions. To solve this issue, we previously developed CRISPRcleanR: a computational method implemented as R/python package and in a dockerized version. CRISPRcleanR detects and corrects biased responses to CRISPR-Cas9 targeting in an unsupervised fashion, accurately reducing false-positive signals while maintaining sensitivity in identifying relevant genetic dependencies. Here, we present CRISPRcleanR ^WebApp , a web application enabling access to CRISPRcleanR through an intuitive interface. CRISPRcleanR ^WebApp removes the complexity of R/python language user interactions; provides user-friendly access to a co

SUBMITTER: Vinceti A 

PROVIDER: S-EPMC9939378 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature