High-throughput combined voltage-clamp/current-clamp analysis of freshly isolated neurons.

ABSTRACT: The patch-clamp technique is the gold-standard methodology for analysis of excitable cells. However, throughput of manual patch-clamp is slow, and high-throughput robotic patch-clamp, while helpful for applications like drug screening, has been primarily used to study channels and receptors expressed in heterologous systems. We introduce an approach for automated high-throughput patch-clamping that enhances analysis of excitable cells at the channel and cellular levels. This involves dissociating and isolating neurons from intact tissues and patch-clamping using a robotic instrument, followed by using an open-source Python script for analysis and filtration. As a proof of concept, we apply this approach to investigate the biophysical properties of voltage-gated sodium (Nav) channels in dor

SUBMITTER: Ghovanloo MR 

PROVIDER: S-EPMC9939380 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature