Diagnosis of T-cell-mediated kidney rejection by biopsy-based proteomic biomarkers and machine learning.

ABSTRACT:

Background

Biopsy-based diagnosis is essential for maintaining kidney allograft longevity by ensuring prompt treatment for graft complications. Although histologic assessment remains the gold standard, it carries significant limitations such as subjective interpretation, suboptimal reproducibility, and imprecise quantitation of disease burden. It is hoped that molecular diagnostics could enhance the efficiency, accuracy, and reproducibility of traditional histologic methods.

Methods

Quantitative label-free mass spectrometry analysis was performed on a set of formalin-fixed, paraffin-embedded (FFPE) biopsies from kidney transplant patients, including five samples each with diagnosis of T-cell-mediated rejection (TCMR), polyomavirus BK nephropathy (BKPyVN), and stable (STA) ki

SUBMITTER: Fang F 

PROVIDER: S-EPMC9939643 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature