Circ_0006220 Contributes to NSCLC Progression through miR-342-3p/GOT2 Axis.

ABSTRACT:

Purpose

Dysregulated circular RNAs (circRNAs) have shown crucial modulatory functions in tumorigenesis, containing non-small cell lung cancer (NSCLC). The purpose of this study was to explore the biological functions and regulatory theory of circ_0006220 in NSCLC.

Methods

Reverse transcription-quantitative polymerase chain reaction and Western blot assay were conducted to measure RNA and protein expression, respectively. A total of 73 cases of NSCLC tumor samples were collected for expression analysis, and A-549 and NCI-H1299 cell lines were used for functional experiments. Cell proliferation was assessed by cell counting kit-8 assay, colony formation assay, 5-ethynyl-2'-deoxyuridine assay, and flow cytometry. Cell apoptosis, motility, and angiogenesis ability were analyzed

SUBMITTER: Tang J 

PROVIDER: S-EPMC9939679 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature