Optogenetic control of beta-carotene bioproduction in yeast across multiple lab-scales.

ABSTRACT: Optogenetics arises as a valuable tool to precisely control genetic circuits in microbial cell factories. Light control holds the promise of optimizing bioproduction methods and maximizing yields, but its implementation at different steps of the strain development process and at different culture scales remains challenging. In this study, we aim to control beta-carotene bioproduction using optogenetics in Saccharomyces cerevisiae and investigate how its performance translates across culture scales. We built four lab-scale illumination devices, each handling different culture volumes, and each having specific illumination characteristics and cultivating conditions. We evaluated optogenetic activation and beta-carotene production across devices and optimized them both independently. T

SUBMITTER: Pouzet S 

PROVIDER: S-EPMC9939774 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature