Phenotypic and genetic alterations of Burkholderia pseudomallei in patients during relapse and persistent infections.

ABSTRACT: The bacterium Burkholderia pseudomallei is the causative agent of melioidosis, a severe tropical disease associated with high mortality and relapse and persistent infections. Treatment of melioidosis requires prolonged antibiotic therapy; however, little is known about relapse and persistent infections, particularly the phenotypic and genetic alterations of B. pseudomallei in patients. In this study, we performed pulsed-field gel electrophoresis (PFGE) to compare the bacterial genotype between the initial isolate and the subsequent isolate from each of 23 suspected recurrent and persistent melioidosis patients in Northeast Thailand. We used whole-genome sequencing (WGS) to investigate multilocus sequence types and genetic alterations of within-host strain pairs. We also inves

SUBMITTER: Seng R 

PROVIDER: S-EPMC9939903 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature